Abstract
While birds are the definitive hosts of nematodes of Eustrongylides spp., other vertebrates, such as fish, amphibians and reptiles, represent intermediate/paratenic hosts. Despite their zoonotic potential, however, the taxonomic characterization of the larval stages of fish parasitic nematodes in the Brazilian Amazon remains limited. The present study aims to describe the morphological and molecular characteristics of Eustrongylides sp. found parasitizing the musculature of Hoplia malabaricus from the Amazon Basin, in the region of Santarém, in the state of Pará, Brazil. In addition to presenting a new geographic record, the present study morphologically and molecularly characterizes Eustrongylides sp. larvae in the Brazilian Amazon using the 18S ribosomal gene. As this nematode can be considered a potential emerging cause of disease, it is important to use molecular markers to improve diagnosis, in addition to describing new species and evaluating the genetic distribution of Eustrongylides populations in the Brazilian Amazon.
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Acknowledgments
The author Lincoln Corrêa would like to thank CAPES/FAPESPA (Project N. 06/2015 – Process n° 88881.160660/2017-01) for financial and other support. G.B. Soares was supported by a doctoral scholarship from the São Paulo Research Foundation (FAPESP) (grant #2017/17531-0). M. I. Muller was supported by a Postdoctoral scholarship from the São Paulo Research Foundation (FAPESP) (grant # 2017/16546–3), and the São Paulo State Research Support Foundation (FAPESP) provided financial support for project 16/22047-7 coordinated by Dr. Edson A. Adriano.
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Fish capture and access to genetic heritage were authorized by the Ministry of the Environment (reference numbers SISBIO n° 44,268–4 and SisGen A0A0058, respectively). The methodology of the present study was approved by the Research Ethics Committee of the Federal University of São Paulo (CEUA n° 2,828,270,219- ID 008453).
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Correa, L.L., Soares, G.B., Müller, M.I. et al. First morphological and molecular characterization of the genus Eustrongylides nematode larvae infecting fish in the Brazilian Amazon. Biologia 78, 2855–2862 (2023). https://doi.org/10.1007/s11756-023-01422-6
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DOI: https://doi.org/10.1007/s11756-023-01422-6