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2020
Received : 24 June 2019 Received in revised: 14 August 2019 Accepted : 31 August 2019 Available online : 25 January 2020 DOI: 10.26655/AJNANOMAT.2020.2.4 This paper deals with the rapid photosensitized biosynthesis of silver nanoparticles using aqueous extract of flowers of Cascuta reflexa. The reaction was carried out in ambient sunlight. The mixing of aqueous solution of silver nitrate and the flower extract shows color transitions from yellow to light brown and finally dark brown colour, indicating the formation of silver nanoparticles. As synthesized the nanoparticles were characterized by various techniques such as UV visible spectroscopy, XRD, FT-IR, TEM. The TEM analysis revealed that the particles were predominantly spherical and size ranging from 20 to 50 nm. The antioxidant properties were tested by FR AP assay method. The antibacterial properties of synthesized Nanoparticles were tested against pathogens such. P.aeruginosa, E.coli, B. subtilus and S.aureus.
2020 •
This paper deals with the rapid photosensitized biosynthesis of silver nanoparticles using aqueous extract of flowers of Cascuta reflexa. The reaction was carried out in ambient sunlight. The mixing of aqueous solution of silver nitrate and the flower extract shows color transitions from yellow to light brown and finally dark brown colour, indicating the formation of silver nanoparticles. As synthesized the nanoparticles were characterized by various techniques such as UV visible spectroscopy, XRD, FT-IR, TEM. The TEM analysis revealed that the particles were predominantly spherical and size ranging from 20 to 50 nm. The antioxidant properties were tested by FR AP assay method. The antibacterial properties of synthesized Nanoparticles were tested against pathogens such. P.aeruginosa, E.coli, B. subtilus and S.aureus.
Biological methods of synthesis have paved way for " greener synthesis " of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. This has motivated an upsurge in research on the synthesis routes that allow better control of shape and size for various nanotechnological applications. In this work, we have explored an inventive contribution for synthesis of silver nanoparticles using catharanthus roseus (Sadabahar), Musa paradisiacal (Plantain flower) and Polygonum odoratum (Coriandium) leaf extract. Synthesized nanoparticles were characterized by various methods such as UV-Vis spectroscopy, SEM and XRD. In addition, antibacterial activity of the synthesized silver nanoparticles was also determined. This new method is rapid time scales for biosynthesis of metallic nanoparticles using environmentally benign natural resources as an alternative to chemical synthesis protocols as reductant for synthesizing silver nanoparticles. 250 | P a g e
South Asian Journal of Research in Microbiology
Biosynthesis and Characterization of Silver Nanoparticles Produced by Plant Extracts and Its Antimicrobial ActivitySolanum tubersum is the fourth most challenging plant in Egypt, affected by several fungi, viral and bacterial diseases. Bacterial and fungal isolates (Brown rot disease (Ralstonia solaniserum), soft root disease (Pectobacterium carotovora) and dry rot disease (Fusarium oxisporum) were collected. The green extracts of silver nanoparticles were prepared by means of aqueous extracts of three wild plants, Physalis peruviana (leaves, red and green fruits) (N1, N2 and N3), Solanum nigrum (fruit) (N4) and Moringa oliefera (leaves) (N5). SEM, TEM, FT-IR and X-RD obtained the characterization of the biosynthesis of silver nanoparticles. The results indicated that nanoparticles were spherical, smooth and the sizes varied between 12 and 33 nm. The activity of the nanoparticle formulations was tested against the bacterial isolates using agar diffusion method and one fungus using mycelial growth method. The results also elucidated that N5 formulation showed a significantly potent antibacterial ...
Aqueous seed extract of Syzygium cumini (SCE) was used for the synthesis of silver (Ag) nanoparticles. The nanoparticles were characterized with the help of UV–vis spectrophotometer, Particle size analyser (PSA), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). UV-Vis spectra of silver nanoparticles shows absorbance peak at 440 nm. The nanoparticles synthesized were relatively spherical in shape with varying sizes. The biosynthesized silver nanoparticles were found to be highly toxic against some human pathogenic bacteria.
The laboratory experiments were conducted to synthesis silver nanoparticles using the leaf extract of Cansjera rheedii J. F. Gmel.The characteristic Plasmon resonance at 430 nm in UV-Vis spectrophotometer confirmed the formation Silver nanoparticles. The FTIR data reveals the possible functional groups of biomolecules involved in bioreduction and capping for efficient stabilization of silver nanoparticles. HRSEM studies revealed that the nanoparticles are spherical in shape and size ranges between 30 and 50 nm. These silver nanoparticles were evaluated for their antibacterial efficacy against the bacteria Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. The Silver nanoparticles inhibited the growth of Pseudomonas aeruginosa and staphylococcus aureus but no inhibition was observed in case of Escherichia coli.
Nanomaterials
Antibacterial and Cytotoxic Potential of Biosynthesized Silver Nanoparticles by Some Plant Extracts2018 •
The silver nanoparticles (AgNPs) have gained attraction of Chemist and Physic’s since last decade and widely accepted in electronics, engineering, nanotechnology, biotechnology, medicine and chemical industries. The green synthesis of silver nanoparticles has been known for their beneficial, eco friendly and less hazardous results as compared to other conventional methods. Based on the literature survey, here we first time report the synthesis and bioactive analysis of (AgNPs) by aqueous extract of leafs of variety of medicinally important plant belonging from family Cupressaceae. During the study, multiple changed was observed for different part of different plants, an intense surface resonance Plasmon band in the UV-visible region at 465 nm. The further studies of formation of AgNPs were confirmed by UV, FTIR, SEM and antimicrobial activity.
International Journal of Biosciences (IJB)
Synthesis of silver nanoparticles from plant extracts and their antimicrobial applicationJournal of AOAC International
Biogenic Synthesis and Characterization of Silver Nanoparticles Using Some Medical Plants and Evaluation of Their Antibacterial and Toxicity Potential2018 •
Background: Silver nanoparticles (AgNPs) are employed in various applications in the areas of catalysis, optoelectronics, detection and diagnostics, antimicrobials, and therapeutics. Objective: The aim of this work was to study the antimicrobial activity of aqueous and methanolic leaf extracts of Thymus vulgaris and Urtica dioica and biologically prepared silver nanoparticles, as single or in combination treatments, against Escherichia coli and methicillin-resistant Staphylococcus aureus isolates. Methods: The minimum inhibitory concentration (MIC) was quantified by using a microdilution method in sterile 96-well microtiter plates. The assessment of the toxicity of AgNP solutions was evaluated on human blood lymphocyte cells. Results: The results of this study revealed that all AgNP solutions have the lowest MIC values against the bacterial isolates in relation with the methanolic and aqueous extract solutions. However, the results showed that the increasing AgNP concentration was a...
International Journal of Pharmacy and Pharmaceutical Sciences
PLANT MEDIATED SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES Original ArticleObjective: The study was aimed to synthesis and characterization of silver nanoparticles from five different herbal plants (Terminalia chebula, Mimusops elengi, Myristica fragrans, Centella asiatica and Hemidesmus indicus). Methods: The qualitative analysis of plant extracts was performed to determine the presence of secondary metabolites. The plant mediated silver nanoparticles were synthesized. The color changed into brown to black color indicating the formation of AgNPs. The characterization of synthesized AgNPs was carried out by different methods such as UV-Vis Spectra, FE-TEM, Particle size analysis, Zeta potential analysis, XRD and FTIR. The antimicrobial activity of synthesized silver nanoparticles also examined against three fungi and bacteria. Results: The UV wave length of AgNPs is from 300 to 450 nm. The average size of AgNPs 581 d.nm, zeta potential is -13.3 mV. The FTIR results show that AgNPs contains the functional groups. In antimicrobial activity of all AgNPs synth...
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